Lecithin-Bound Iodine Prevents Disruption of Tight Junctions of Retinal Pigment Epithelial Cells under Hypoxic Stress

Figure 3

Hypoxia-induced tight junction disruption is also suppressed by blockade of MCP-1 or CCL-11. Exposure on ARPE-19 cells to CoCl₂ causes TJ disruption ((d) arrow head). When the cells were pretreated with anti-MCP-1 or CCL-11 antibody, these changes were reduced in the ZO-1 pattern compared to that of the control ((e), (f)). Thus, blockade of MCP-1 and CCL-11 can protect conformation of TJs from hypoxic stress. CCL-11, Chemokine (C-C Motif) Ligand 11; IL, interleukin; LBI, lecithin-bound iodine; MCP-1, monocyte chemoattractant protein-1; TJs, tight junctions; ZO-1, zonular occludin-1.

(a)  

(b)

(c)

(d)

(e)

(f)