Application of Metagenomic Next-Generation Sequencing in the Diagnosis of Infectious Keratitis

Table 1

Comparison of detection methods for fungus and bacteria.

Microorganisms	Detection methods	Sample case (n)	Positive case (n)	Positive rate (%)
Fungi	SC	143	99	69.2
	CM	137	113	82.5
	mNGS	70	51	72.9
	PS	109	40	36.7
		60.436
		<0.001
Bacteria	SC	67	46	68.7
	mNGS	38	34	89.5
		5.792
		0.016

SC, secretion culture; CM: corneal laser confocal microscopy; PS: pathological section; mNGS, metagenomic next-generation sequencing. For fungus: the positive rate of CM was higher than that of SC ( = 6.681, ), but no difference was observed between CM and mNGS ( = 2.608, ), the positive rate of SC was similar to mNGS ( = 0.297, ), and the positive rate of PS was the lowest (all ). For bacteria, 21 SC cases were negative but were detected using mNGS. Four cases of mNGS were negative but were detected using SC.