Retaining Residual Ovarian Tissue following Ovarian Failure Has Limited Influence on Bone Loss in Aged Mice

Figure 2

Trabecular microarchitecture in aged VCD-treated and ovariectomized mice. Aged C57BL/6Hsd female mice (9–12 months old) were dosed daily for 15 days with sesame oil (vehicle; CON) or VCD (160 mg/kg) or were ovariectomized on d35 after the start of the experiment. Trabecular bone microarchitecture of the fifth vertebral body was assessed by $\mu$CT on samples collected at 5-month postovarian failure (age: 18.8 months; d181) as described in Section 2. Data are represented as mean $\pm$ SEM ($n=4\text{–}6$). Significance was set at $P<.05$. The following variables were measured: bone volume fraction (BV/TV%), trabecular thickness (TbTh, $\mu$m), trabecular separation (TbSp, $\mu$m), trabecular number (TbN, 1/mm), connectivity density (ConnD, 1/mm³), and structure model index (SMI). (*) indicates $P<.05$ versus CON; ($\#$) indicates $P<.05$ versus VCD. Trends are represented by bars indicating the corresponding P-value.

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