Recent Advances in Molecular Technologies and Their Application in Pathogen Detection in Foods with Particular Reference to Yersinia
Table 2
Comparison of major next generation DNA sequencing technologies and conventional sequencing.
Platform
Application
Sequencing chemistry
Read length (bases)
Throughput per run (Gb)
Read per run (million)
Throughput per 24 hr (Gb)
Raw accuracy Range (%)
Cost Pe Mb ($)
ABI 3730
(1) Complement de novo assemblies for high-quality assembly of complex genomes; (2) Custom sequencing (3) Targeted resequencing for polymorphism discovery and genotyping
Sanger Dideoxy
800
0.00008
0.000096
0.00064
99.0 to 99.999
4000
ABI SOLID 5500
(1) Whole genome SNP discovery; (2) Transcriptome assembly and expression profiling; (3) Whole methylome resequencing
Sequencing by ligation
310
5167
45
99.0 to 99.9
0.05
Illumina HiSeq
(1) Whole genome SNP discovery; (2) Transcriptome assembly and expression profiling; (3) Whole methylome resequencing; (4) Bacterial and megaplasmid de novo assembly
Sequencing by synthesis
600
6000
75
96.2 to 99.7
0.02
Life Technologies Ion Torrent
(1) Whole methylome resequencing; (2) Bacterial and megaplasmids de novo assembly; (3) Sequencing quality control; (4) Sequencing requirement lower complexity
pH meter
200
0.2
1
2.4
>99.0
0.5
Roche 454
(1) De novo assemblies of complex genomes; (2) Metagenomics; (3) Analysis of large structural variations
