Virulence Plasmid (pYV)-Associated Expression of Phenotypic Virulent Determinants in Pathogenic Yersinia Species: A Convenient Method for Monitoring the Presence of pYV under Culture Conditions and Its Application for Isolation/Detection of Yersinia pestis in Food

Figure 3

Detection of pYV in cells recovered from red pinpoint colony and subcultured in brain heart infusion broth at 28°C by PCR assay targeting virF gene of pYV. The primer pairs (5′-TCATGGCAGAACAGCAGTCAG-3′ and 5′-ACTCATCTTACCATTAAGAAG-3′) for detection of the virF gene (430- to 1020-nucleotide region) amplified a 591 base-pair (bp) product from the virulence plasmid. Lane M, 50–1,000 bp ladder marker; lane 1 showing the absence of 591-bp product in Y. pestis; lanes 2 and 3 showing the presence of 591-bp product in Y. enterocolitica and Y. pseudotuberculosis, respectively.