Comparative Analyses of Exoproteinases Produced by Three Phytopathogenic Microorganisms

Table 2

Exoproteinase activity (U, nmol/mg of substrate per minute) of the oomycete P. infestans, fungi R. solani, and F. culmorum in hydrolysis of p-nitroanilides of amino acids and tripeptides (substrate concentration was 0.5 mM).

Substrate	Exoproteinases of P. infestans	Exoproteinases of R. solani	Exoproteinases of F. culmorum
BAPNA	4.2 ± 0.17	2.7 ± 0.07	1.9 ± 0.06
Z-AALPNA	2.1 ± 0.08	0.5 ± 0.02	6.7 ± 0.23
Suc-FPNA	0 ± 0.00	0 ± 0.00	0.4 ± 0.01
Suc-GGFPNA	0 ± 0.00	0 ± 0.00	0.5 ± 0.01
LPNA	0 ± 0.00	0.1 ± 0.01	1.1 ± 0.03
Ac-AAAPNA	0 ± 0.00	0.1 ± 0.01	1.0 ± 0.02