Design and Construction of a Single-Tube, LATE-PCR, Multiplex Endpoint Assay with Lights-On/Lights-Off Probes for the Detection of Pathogens Associated with Sepsis

Figure 11

The dashed line shows the fluorescent contour for S. epidermidis plus 35,000 copies of human genomic DNA. As shown, the SE contour is not impacted by addition of human gDNA as it matches the contour for SE alone (-°-). The contour for human gDNA is shown by the solid black line.