Proof of Principle for a Real-Time Pathogen Isolation Media Diagnostic: The Use of Laser-Induced Breakdown Spectroscopy to Discriminate Bacterial Pathogens and Antimicrobial-Resistant Staphylococcus aureus Strains Grown on Blood Agar

Table 2

Mutations detected in MM66 and SH1000-1 by comparative genome sequencing.

Strains	SACOL loci^a	Gene	Function	SNP position^a	Amino acid change^a
SH1000-1	Intragenic
	SACOL0593	fusA	Elongation factor G	C^617228→ T^617228	H^457→ Y^457
	SACOL0358			A^371671→ T^371671	N^36→ I^36
				T^317672→ A^371672	N^36→ I^36
				G^371676→ A^371676	E^37→ K^37
				A^371685→ C^371685	K^40→ Q^40
	MM66-4	Intragenic
SACOL1883-			Hypothetical protein	C^1938819→ T^1938819
TRNA-ser			tRNA-ser	T^1938825→ A^1938825
SACOL1947-			Hypothetical protein	C^2010604→ T^2010604
SACOL1948			Hypothetical protein	A^2010605→ G^2010605
SACOL2575-			Putative aromatic; Amino transferase	A^2638759→ T^2638759
SACOL2576		crtN	Squalene synthase	C^2638762→A^2638762
Intragenic
SACOL1690		apt	Adenine phosphoribosyl-; transferase	C^1721075→T^1721075	A^57→ V^57
SACOL0020		yycG	Sensory box histidine; kinase	A^26449→ G^26449	K^263→ E^263

SNP: single nucleotide polymorphism.
aBased on loci numbers, nucleotide positions, and amino acid residues in NCBI Genbank database S. aureus strain COL reference genome.