Effective Concentration and Detection of Cryptosporidium, Giardia, and the Microsporidia from Environmental Matrices
Table 2
Pathogen detection through Method 1623 and CFD + qPCR in environmental samples.
Environmental samples
Method 1623
CFD + qPCR
Sample
Date
Volume (L)
C. parvum
a
G. intestinalis
a
(oo)Cysts per Lb
Vol. (L)c
Pathogen(s) detectedd
Number of pathogense
Pathogens per Lf
City of Auburndale WWTF
10/12/2010
50.0
8
572
0.2/11
21
G. intestinalis and E. bieneusi
122/634
6/30
Intake FL6290327 DLTWTF
10/12/2010
48.5
0
0
0/0
8.4
0
0
0/0
Lake Wales Utilities Sam P. Robinson Reclaimed WTP
11/3/2010
50.0
0
0
0/0
8
E. intestinalis and E. hellem
312
0/0
City of St. Petersburg 401 ASRWRF Inj. Well
12/13/2010
50.3
21
78
0.4/1.5
10.4
E. bienusi
1286
124
Intake FL6290327 DLTWTF
1/11/2011
48.8
0
2
0/0.04
5
0
0
0
Lake Wales Utilities Sam P. Robinson Reclaimed WTP
1/12/2011
40.5
89
291
2/7
4
G. intestinalis
105
26
City of St. Petersburg 401 ASRWRF Inj. Well
2/1/2011
50.3
24
17
0.5/0.3
19.5
E. bieneusi
754
39
City of St. Petersburg 764 ASRWRF B-4 Mon. Well
2/1/2011
50.4
0
0
0/0
19.3
E. bieneusi
538
28
City of Tampa Howard F Curren AWTP
2/2/2011
50.3
2
4
0.04/0.08
22
0
0
0
City of St. Petersburg MW401 ASRWRF B-10 Mon. Well
2/15/2011
50.7
0
0
0/0
20
E. intestinalis and E. hellem
677
34
Intake FL6290327 DLTWTF
2/2/2011
50.3
0
2
0/0.04
15.5
E. hellem
1515
98
Number of oocysts/cysts per detected in sample.
bApproximate number of oocysts/cysts per liter.
cSample volumes remaining after Method 1623 analysis.
dPathogens detected in sample using hybridization probes and verified via downstream sequencing.
eEstimated number of pathogens in sample (number of pathogens calculated × total volume of gDNA)/(volume of gDNA analyzed).
fApproximate number of respective pathogens per liter.
