Differential Regulation of Escherichia coli fim Genes following Binding to Mannose Receptors

Figure 1

Quantitative determination of mRNA regulation by LD-RT-PCR analysis of cDNAs of strain NU149 cells mixed with plain Sepharose beads (−) or mannose-coated Sepharose beads (+) for 10, 25, 60, or 120 min. The FimB1/FimB2, FimE1/FimE2, and EcFtsZ1/EcFtsZ2 primer pairs were used to amplify serially twofold diluted cDNAs and targeted fimB (379 bp product), fimE (392 bp product), and ftsZ (302 bp product) transcripts, respectively. All PCR products were electrophoresed on 1.5% agarose gels. The following dilutions of cDNAs were used: undiluted (lane 1), 1/2 (lane 2), 1/4 (lane 3), 1/8 (lane 4), 1/16 (lane 5), 1/32 (lane 6), 1/64 (lane 7), 1/128 (lane 8), and 1/256 (lane 9). The data represent at least three separate runs.