Differential Regulation of Escherichia coli fim Genes following Binding to Mannose Receptors

Figure 2

Effects of fimB transcription in strain NU149/pWS145-38 containing a fimB-lux transcriptional fusion grown in a pH 5.5 or 7 environment mixed with plain Sepharose beads, mannose-coated Sepharose beads, of mannose-coated Sepharose beads with 2% free mannose (wt/vol) added. Columns represent NU149 grown in pH 5.5 LB mixed with mannose-coated Sepharose beads (black column), plain Sepharose beads (white column), or mannose-coated Sepharose beads plus 2% free D-mannose (gray column) as well as NU149 grown in pH 7 LB mixed with mannose-coated Sepharose beads (left striped column), plain Sepharose beads (white dots column), or mannose-coated Sepharose beads plus 2% free D-mannose (right striped column). The RLU/CFU were calculated by using a luminometer to measure luminescence, subtracting out the background, and then dividing by viable counts. The data represents that the means standard deviations are indicated from at least three separate runs. equals < 0.05 and equals < 0.0001.