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Journal of Pharmaceutics
Volume 2013 (2013), Article ID 495814, 6 pages
http://dx.doi.org/10.1155/2013/495814
Research Article

Determination of Zalcitabine in Medicaments by Differential Pulse Voltammetry

1Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil
2Department of Chemistry, Pontifícia Universidade Católica, 225 Rua Marquês de São Vicente, 22453-900 Rio de Janeiro, RJ, Brazil

Received 5 February 2013; Revised 13 March 2013; Accepted 13 March 2013

Academic Editor: Antonio Ruiz Medina

Copyright © 2013 Katia Christina Leandro et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The zalcitabine (ddC) has been extensively used in the treatment of HIV patients due to its antiretroviral activity. The quality control of this active principle in medications is of outstanding importance to public health. The principal objective of the current study was the development of an alternative analytical methodology for the zalcitabine determination using a voltammetric process. The zalcitabine gives a reduction peak (at  V versus Ag/AgCl) at the hanging mercury drop electrode (HMDE). The differential pulse voltammetric response is evaluated with respect to the scan rate (20 mV/s), pulse amplitude (50 mV), support electrolyte (Clark-Lubs buffer), pH (2.0), and other variables. The response is linear over the 10.0 to 28.0 mg/L (47 to 133 μM) concentration range, and the detection limit is 2.08 mg/L. The validation of this method was realized using a governmental Brazilian document (Inmetro, 2007) and the results are reported for medication drugs.