Optimization of Cultural Conditions for Production of Extracellular Polymeric Substances (EPS) by Serpentine Rhizobacterium Cupriavidus pauculus KPS 201

Table 1

Effect of various carbon sources on growth and production of EPS by Cupriavidus KPS 201 cells.

Carbon sources	Growth, Log no. of cells/mL	EPS, µg/mL	Final pH
Gluconate	9.246±0.02	105.63±1.21	7.62±0
Glutamate	9.320±0.03	132.30±5.89	8.23±0
Acetate	8.860±0.04	38.44±5.23	8.09±0.05
Pyruvate	9.106±0.02	109.13±3.58	7.96±0.01
Fumarate	9.178±0.02	94.76±3.17	8.45±0.01
Malate	7.287±0.02	0	7.12±0.01
Malonate	9.158±0.04	16.18±4.28	8.45±0.01
Formate	7.348±0.06	0	7.53±0.01
Citrate	9.174±0.09	63.70±5.35	8.35±0.03
Succinate	9.226±0.03	99.69±3.09	8.45±0.01

Cells were grown in Tris-minimal broth supplemented with respective carbon sources used as sodium salts at 0.4% concentration for 72 h.