Interactions between Leishmania braziliensis and Macrophages Are Dependent on the Cytoskeleton and Myosin Va

Figure 4

Integrity of the cytoskeleton influences the interaction between L. braziliensis and macrophages. Adherent Raw 264.7 macrophages were incubated for 10 min with (a) fresh DMEM, (b) 5 𝜇M latrunculin A to disrupt actin filaments, or (c) 5 𝜇M nocodazole to disrupt microtubules before exposure for 30 min to parasites that had been previously marked with CFSE. The panels are representative fields of colour-combined fluorescent images displaying parasites in green and F-actin (phalloidin staining) in red. Scale bars indicate imaging for all panels at 63x magnification.

(a)

(b)

(c)