Interactions between Leishmania braziliensis and Macrophages Are Dependent on the Cytoskeleton and Myosin Va
Figure 5
Internalisation of L. braziliensis requires myosin Va. The role of myosin Va was examined by observing the distribution of endogenous myosin Va in relation to parasites and the effect of the expression of a dominant negative tail domain fused to eGFP. Adherent Raw 264.7 macrophages were incubated with fluorescently marked parasites for 30 min before fixation, preparation and imaging by confocal microscopy. (a) shows endogenous myosin Va stained by an antibody (green) and parasites (red). (b) shows the myosin Va tail (green) and parasites (red). (c) shows mock-transfected macrophages by phase microscopy overlaid with a fluorescent image of parasites (red). Scale bars indicate imaging for all panels at 63x magnification.
