Comparison of Protective Immune Responses to Apicomplexan Parasites
Figure 1
Developing and mature micro- and macrogametes and oocysts harvested from the infected chicken intestine 134 h post infection (p.i.) and double-labelled with a monoclonal antibody raised to antigens confound to WFB1s (E1D8) [107], and a polyclonal affinity purified gametocyte antigens Emgam56, Emgam82, and Emgam230, amongst other molecules (anti-APGA) specific to molecules contained within the wall-forming bodies (1 and 2) [53] and visualised with fluorescein isothiocyanate (green) or revealed with rhodamine-conjugated goat anti-mouse IgG secondary antibody (red). Counterstained with DAPI. Abbreviations: N, nucleus; SO, sporulated oocysts; SW, sporocyst wall; US, unsporulated oocyst; WFB1, wall-forming body type 1; WFB2, wall-forming body type 2. Bars represent 5 μm.
