Research Article
Development of Eimeria nieschulzi (Coccidia, Apicomplexa) Gamonts and Oocysts in Primary Fetal Rat Cells
Table 1
Number and type of experiments performed in this study.
| Cells | Coating | Cell growth | Max. parasite development |
| IPEC-J2 [2] | Uncoated | Monolayer | Sch II/Mz II | ST [2] | Uncoated | Monolayer | Sch II/Mz II | VERO [>2] | Uncoated | Monolayer, later multilayer | Sch II/Mz II | IEC6 [>2] | Uncoated | Monolayer | Sch II/Mz II | Prim.intestine [3] Prim.intestine [1] Prim.intestine [1] | Fibronectin Gelatin Uncoated | Monolayered fibroblast, degrading cell conglomerates, no growing organoids | Sch II/Mz II | Primary liver [2] | Fibronectin | Monolayer | Sch II/Mz II | Primary kidney [2] | Fibronectin | Monolayer | Sch II/Mz II | Primary fetal mix [3] | Fibronectin | Monolayer and cell conglomerates in two experiments (1., 2.) no growing organoids in one experiments (3.) growing crypt-like organoids | (1.) Sch II/Mz II (2.) Sch II/Mz II (3.) Mag/Ooc | Primary fetal mix [2] | Gelatin | Monolayer and cell conglomerates, in one experiment no organoids (1.) in one experiment growing crypt-like organoids (2.) | (1.) Sch II/Mz II (2.) Mag/Ooc. | Primary fetal mix [1] | Matrigel | Monolayer and growing organoids | Mag/Ooc | Primary fetal mix (3) | Uncoated | Monolayered fibroblast, degrading cell conglomerates, no growing organoids | Sch II/Mz II |
|
|
[number of experiments]; (experiment number) Sch II: schizont 2nd generation, Mz II merozoite 2nd generation, Mag: macrogamont, Ooc: oocyst.
|