Research Article
Improved Method for the Detection and Quantification of Naegleria fowleri in Water and Sediment Using Immunomagnetic Separation and Real-Time PCR
Table 1
IMS recovery efficiency for
N. fowleri seeded into 5 mL of water (
).
| | Genotype (strain) | Water type | Cyst stage (average ± SD) | Trophozoite stage (average ± SD) |
| | I (CDC:V212) | Amended DI | % | % | | Lake* | % | % | | II (CAMP) | Amended DI | % | % | | Lake | % | % | | III (CDC:V515) | Amended DI | % | % | | Lake | % | % | | IV (ATCC 30462) | Amended DI | % | % | | Lake | % | % | | All 4 genotypes | Amended DI | % | % | | Lake | % | % |
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GA lake water filtered through a 2.7 m cellulose acetate filter.
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