Table 3: N. fowleri detection in seeded 1 L lake water samples.

Seed levelDirect PCR without culture Culture followed by PCR confirmation
IMSNo IMSIMSNo IMS
CT Pos no.CT # PosCT Pos no.CT # Pos

1439.1138.01Negative038.91
6338.5 340.5 232.8 134.6 2
38835.1 436.7 427.8 438.3 1
4,90031.9 433.2 428.9 435.7 4
11,30029.83429.96423.99434.874
129,00027.87428.19425.38431.574

Number of positive results out of four replicate tests (all conditions were repeated four times).