Research Article
Improved Method for the Detection and Quantification of Naegleria fowleri in Water and Sediment Using Immunomagnetic Separation and Real-Time PCR
Table 5
Estimated concentration of N. fowleri in water and sediment samples.
| Sample ID | No. of amebas seeded | Average CT value | Estimated concentration (amebas/L) |
| GA water | 14 | 39.1 | 8 | GA water | 63 | 38.5 | 15 | GA water | 388 | 35.1 | 183 | GA water | 4,900 | 31.9 | 1,224 | GA water | 11,300 | 29.8 | 5,902 | GA water | 129,000 | 27.9 | 18,620 | FL5 water | NA | 40.6 | 2.7 | FL6 water | NA | 38.2 | 15 | MN9 sediment | NA | 38.3 | 13 | WB saline control | 9,800 | 28.8 | 7,900 | WB saline control | 37,000 | 27.8 | 15,000 |
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