Partial Purification of Integral Membrane Antigenic Proteins from Trypanosoma evansi That Display Immunological Cross-Reactivity with Trypanosoma vivax
Figure 5
Separation of the SSDS fraction using sedimentation on a linear 10–30% sucrose gradient. A 200 μL aliquot of the T. evansi SSDS sample was loaded on a 4 mL linear 10–30% sucrose gradient. Fractions (80 μL) were collected through the bottom of the tube following ultracentrifugation. (a) SDS-PAGE analysis of the resulting fractions; shown is the Coomassie blue staining. (b) Western blot developed with serum B-173 (Table 1). M = protein molecular weight markers.
