Could kDNA-PCR in Peripheral Blood Replace the Examination of Bone Marrow for the Diagnosis of Visceral Leishmaniasis?
Table 1
Results of clinical, epidemiological, and laboratorial diagnosis of visceral leishmaniasis in Group 1 (true VL cases) (1).
Number of samples (), age (), and sample entrance (SE)
Comorbidities (CM)
Clinical and laboratorial manifestations
Epidemiological data (place of origin)
Laboratorial investigation for VL (gold standard)
Laboratorial investigation for VL (tests)
Parasitological techniques in bone marrow (BM)
Serological technique (rK39) in peripheral blood (PB)
Parasitological techniques in peripheral blood (PB)
Molecular technique (kDNA) in bone marrow (BM) and peripheral blood (PB)
BM-S
BM-C
PB-rK39
PB-S
PB-C
BM-kDNA
PB-kDNA
: 1.1 : 19 SE: 07/30/2009
Systemic lupus erythematosus
Hepatosplenomegaly and pancytopenia
Bahia Sister and dog with VL
Neg
Neg
Pos
Neg
Neg
Pos
Pos
: 1.2 : 20 SE: 05/25/2011
Systemic lupus erythematosus
VL with death Hepatosplenomegaly and pancytopenia
Bahia
Pos
Neg
Pos
Neg
Neg
Pos
Pos
: 2.1 : 31 SE: 08/14/2009
HIV
Hepatosplenomegaly and fever
Mato Grosso
Pos
Neg
Neg
Neg
Neg
Pos
Pos
: 2.2 : 33 SE: 02/22/2011
HIV
Hepatosplenomegaly and fever
Mato Grosso
Pos
Neg
Neg
Neg
Neg
Pos
Pos
: 2.3 : 33 SE: 03/11/2011
HIV
Hepatosplenomegaly and fever
Mato Grosso
Pos
Neg
Neg
Neg
Neg
Pos
Pos
: 2.4 : 33 SE: 03/30/2011
HIV
Hepatosplenomegaly and fever
Mato Grosso
Pos
Neg
Neg
Neg
Neg
Pos
Pos
: 3 : 26 SE: 09/18/2009
HIV
VL severe with death Hepatosplenomegaly and fever
São Paulo
Pos
Neg
Neg
Neg
Neg
Neg
Pos
: 4 : 69 SE: 12/07/2009
Hepatosplenomegaly
Ceará
Neg
Neg
Pos
Neg
Neg
Pos
Pos
: 5 : 30 SE: 02/01/2010
No
Hepatosplenomegaly, fever, and weight of loss
Bahia
Neg
Neg
Pos
Neg
Neg
Pos
Pos
: 6 : 74 SE: 04/16/2010
No
VL with death Pancytopenia and fever
Minas Gerais
Pos
Pos
Pos
Neg
Neg
Pos
Pos
: 7 : 40 SE: 11/16/2010
No
Splenomegaly, plateletopenia, anaemia, and fever
Pernambuco
Pos
Pos
Pos
Neg
Neg
Pos
Pos
: 8 : 29 SE: 05/11/2011
Schistosomiasis
Hepatosplenomegaly and fever
Bahia
Pos
Pos
Pos
Pos
Neg
Pos
Pos
: 9 : 65 SE: 06/28/2011
Hemophagocytic syndrome
Hepatosplenomegaly, fever, and pancytopenia
Bahia
Pos
Pos
Pos
Pos
Neg
Pos
Pos
: 10 : 53 SE: 08/23/2011
Schistosomiasis hanseniasis
Pancytopenia
Alagoas
Pos
Pos
Pos
Pos
Neg
Pos
Pos
: 11 : 33 SE: 08/23/2011
No
Hepatosplenomegaly and fever
Bahia
Pos
Pos
Pos
Neg
Neg
Pos
Pos
: 12 : 4 SE: 08/16/2011
No
Hepatosplenomegaly, fever pancytopenia, anaemia, weight loss, and lymphadenomegaly
Mato Grosso
Neg
Neg
Pos
Neg
Neg
Pos
Pos
: 13 : 2 SE: 08/25/2011
No
Hepatosplenomegaly, fever, and pancytopenia
Piauí
Pos
Pos
Pos
Pos
Neg
Pos
Pos
: 14 : 48 SE: 08/29/2011
No
Hepatosplenomegaly, fever, and pancytopenia
Bahia
Pos
Neg
Pos
Neg
Neg
Pos
Pos
: 15 : 45 SE: 11/11/2011
No
Hepatosplenomegaly, fever, and weight loss
Bahia
Pos
Pos
Pos
Neg
Neg
Pos
Pos
: 16 : 20 SE: 03/27/2011
No
Hepatosplenomegaly and fever
Bahia
Pos
Neg
Pos
Neg
Neg
Pos
Pos
BM-S: smear with sample from bone marrow; BM-C: culture with sample from bone marrow; PB-S: smear with sample from peripheral blood; PB-C: culture with sample from peripheral blood; PB-rK39: rK39 with sample from peripheral blood; BM-kDNA: kDNA PCR in sample from bone marrow; PB-kDNA: kDNA PCR in sample from peripheral blood; pos: positive; neg: negative.