Multifunctional Thioredoxin-Like Protein from the Gastrointestinal Parasitic Nematodes Strongyloides ratti and Trichuris suis Affects Mucosal Homeostasis

Figure 6

(a) Exposure of 3D-cocultures to Trichuris suis (Ts) extract. Culture supernatants were harvested after 24 h, 48 h, and 72 h. The release of inflammatory (TNF-α), anti-inflammatory (IL-10), and -related cytokines (IL-22, TSLP) was analyzed in a 3D-cell culture model. Representative results of at least three independent experiments are shown as median. (b) Exposure of 3D-cocultures to SrTrx- and TsTrx-lp or medium (NC). Culture supernatants were harvested after 24 h (A), 48 h (B), and 72 h (C). The release of inflammatory (TNF-α), anti-inflammatory (IL-10), and -related cytokines (IL-22, TSLP) was analyzed in a 3D-cell culture model. Representative results of at least three independent experiments are shown. Significant increase of all measured cytokines compared to NC (). ; .

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