Multifunctional Thioredoxin-Like Protein from the Gastrointestinal Parasitic Nematodes Strongyloides ratti and Trichuris suis Affects Mucosal Homeostasis

Figure 7

Chemotactic activity of the Trx-lp from S. ratti (a) and T. suis (b) for monocytic THP-1 cells. The chemotactic activity of both proteins for THP-1 cells was investigated by using Boyden chambers. Different concentrations of the Trx-lps were added to the lower compartment of the chemotactic chambers. Protein concentrations per 100 μL of 3 ng, 30 ng, 300 ng, and 1 μg showed SrTrx-lp and TsTrx-lp have the greatest chemotactic activity at 3 ng. Chemotaxis buffer (NC) and THP-1 media (RPMI + FCS) were included as negative control (random cell migration), while LPS was used as positive control. All used Trx-lp concentrations led to significant higher cell migration than the negative controls ().

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