Multifunctional Thioredoxin-Like Protein from the Gastrointestinal Parasitic Nematodes Strongyloides ratti and Trichuris suis Affects Mucosal Homeostasis

Figure 8

Percentage closure of the Caco-2 wound gap after 24 h (a), 48 h (b), 72 h (c), and 96 h (d). In general, the gap was narrowed by approx. 10–15% every day adding no stimulus. As a negative control, cells were observed without any stimulus (NC) and with LPS (1 μg). As a positive control, epidermal growth factor (EGF) was used, whereat 10 ng fostered wound healing the best. SrTrx-lp and TsTrx-lp were tested at different concentrations (3 ng, 30 ng, 300 ng, 1 μg, 10 μg, and 25 μg–each per 500 μL), here at 300 ng represented as the best wound healing promoting concentration. The wound healing process was highly significantly promoted by EGF and TsTrx-lp () as well as significantly promoted by SrTrx-lp ().

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