The dynamic hybridization resulted in a decrease in hybridization time. In the static hybridization, the biosensor was hybridized without or with miRNAs extracted from MCF-7 cells in the hybridization solution at for 1 hour, 6 hours, and 12 hours before detection, respectively. For the dynamic detection, the hybridization of biosensor and target miRNAs was monitored in real time without the preincubation with target miRNAs.
