Quartz Crystal Microbalance as a Sensor to Characterize Macromolecular Assembly Dynamics
Figure 18
QCM-D response of overtones as a function of time for protein adsorption and subsequent cross-linking. (a) Exposure of the methyl-terminated surface to a buffer solution containing 25 mg/mL Mefp-1 in 0.1 M acetate buffer (0.75 M NaCl, pH 5.5), followed by exchange of the protein solution for a pure buffer solution. The cross-linking behavior of Mefp-1 was subsequently followed by addition of the same buffer solution containing 1 mM NaIO4. (b) Corresponding dissipation as a function of time for experiment shown in (a). (c) The best fit between the viscoelastic model (obtained using -Soft software(-Sense AB), and the experimental data shown in Figure 17(a). (d) Change in the effective thickness with respect to time obtained from the fit shown in (c). Reprint from [42].
