601704.fig.001
Figure 1: SPR sensorgram showing sensor response to (1) PBS at pH 7.4 with 200 μg/mL BSA for 10 minutes, followed by (2) 10% urine in PBS at pH 7.4 with 200 μg/mL BSA for 30 minutes. The spike in the data at 10 minutes is an unexpected response and does not stabilize until 20 minutes after injection. A 1 nm SPR wavelength shift at 750 nm to 751 nm represents a protein surface coverage of ~161 pg/mm2.