601704.fig.003a
(a)
601704.fig.003b
(b)
601704.fig.003c
(c)
601704.fig.003d
(d)
Figure 3: Normalized reference compensated SPR sensorgrams showing sensor responses for 2 μg/mL of anti-TTX antibody incubated with various concentration of TTX in (a) 100 mM sodium acetate buffer at pH 4.5 with 50 mM NaCl and 200 μg/mL BSA (SAB), (b) SAB at pH 4.5 with 10% pufferfish liver extract, (c) SAB at pH 4.5 with 10% pufferfish muscle extract, and (d) SAB at pH 4.5 with 10% urine. Samples containing complex media were normalized to an antibody reference in buffer. The data shown is the average from three detection cycles.