Measurement of Hepatitis B Surface Antigen Concentrations Using a Piezoelectric Microcantilever as a Mass Sensor

Table 2

Procedures and time requirements in detection process of HBsAg.

	Procedure	Time (min)
Sensor cleaning	UV cleaning	30
	2-Propanol cleaning	10
	Ethanol cleaning	30
	Methanol cleaning	10
	DI water rinsing	5
Protein A/G-SH	Protein A/G-SH (5 μg/mL)	40
	PBS rinsing	5
	DI rinsing	5
	N2 gas dry	1
BSA	BSA (0.01%)	30
	PBS rinsing	5
	DI rinsing	5
	N2 gas dry	1
Anti-HBsAg	Anti-HBsAg (25 μg/mL)	30
	PBS rinsing	5
	DI rinsing	5
	N2 gas dry	1
Control	PBS	10
	DI rinsing	5
	N2 gas dry	1
	AFP	10
	PBS rinsing	5
	DI rinsing	5
	N2 gas dry	1
HBsAg	HBsAg	10
	PBS rinsing	5
	DI rinsing	5
	N2 gas dry	1