Ink-Jet Printing: Perfect Tool for Cantilever Array Sensor Preparation for Microbial Growth Detection

Figure 4

SEM images of cantilevers demonstrating accuracy of drop positioning and cell distribution after 24 h incubation at growth conditions. The width of cantilevers is 100 μm in each case. Ten drops of cell suspension were deposited at the front end of the cantilevers. (a) Imprints of series of droplets on agarose-coated cantilever, after growth of P. aeruginosa. (b) Ten perfectly spotted drops of S. aureus (2.92×10⁸ cells/mL, 10% LB) cells on an agarose-coated cantilever. One spot was deliberately deposited off-position. (c) S. aureus (3.28×10⁸ cells/mL, LB) growth on cantilever surface. (d) P. aeruginosa (1.84×10⁸ cells/mL, 10% LB) growth on cantilever surface. Cells are growing over the clamping point of the cantilever.

(a)

(b)

(c)

(d)