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Journal of Sensors
Volume 2012 (2012), Article ID 696247, 7 pages
http://dx.doi.org/10.1155/2012/696247
Research Article

Development of Galactose Biosensor Based on Functionalized ZnO Nanorods with Galactose Oxidase

Division of Physical Electronic and Nanotechnology, Department of Science and Technology, Linköping University, Campus Norrköping, 60174 Norrköping, Sweden

Received 31 December 2011; Revised 28 April 2012; Accepted 13 May 2012

Academic Editor: P. Siciliano

Copyright © 2012 K. Khun et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The fabrication of galactose biosensor based on functionalised ZnO nanorods is described. The galactose biosensor was developed by immobilizing galactose oxidase on ZnO nanorods in conjunction with glutaraldehyde as a cross-linker molecule. The IRAS study provided evidence for the interaction of galactose oxidase with the surface of ZnO nanorods. The electromotive force (EMF) response of the galactose biosensor was measured by potentiometric method. We observed that the proposed biosensor has a linear detection range over a concentration range from 10 mM to 200 mM with good sensitivity of 8 9 . 1 0 ± 1 . 2 3  mV/decade. In addition, the proposed biosensor has shown fast time response of less than 10 s and a good selectivity towards galactose in the presence of common interferents such as ascorbic acid, uric acid, glucose, and magnesium ions. The galactose biosensor based on galactose oxidase immobilized ZnO nanorods has a shelf life more than four weeks.