A Coumarin-Based Fluorescence Probe for Selective Recognition of Cu²⁺ Ions and Live Cell Imaging

Figure 2

Fluorescence emission spectra of L (10 μM) upon addition of various concentrations of Cu²⁺ ions (0~65 μM) in CH₃CN/HEPES (3 : 2, ) buffer solution. The excitation wavelength was 438 nm. Inset: fluorescence intensities at 487 nm of the L as a function of Cu²⁺ ion concentrations (0~65 μM).