Characterization of Ex Vivo Expanded Tumor Infiltrating Lymphocytes from Patients with Malignant Melanoma for Clinical Application
Figure 3
Functional capacity. Determination of TAA peptide-specific populations in TIL pre- and post-REP. Results from Elispot detection of INFγ in three patients exemplifies the general tendency of specificity retention, decline, and/or increase as a consequence of unspecific stimulation during expansion procedures, (a) Autologous tumor cell lines were available from four patients, and all showed TIL with antitumor activity by measuring INFγ in Elispot. Representative results of TIL from three patients show retained tumor-specific activity. However, in patient MM + 16 we found a component of unspecific NK/LAK cell activity, which seemed to decline after REP, (b). Example of preserved lytic capacity of TIL from MM 11 after REP. Both cultures show specific killing of two established autologous tumor cell lines. Interestingly, Tumor 1 seems more immunogenic than Tumor 2, (c).
