Journal of Skin Cancer

Research Article

Interleukin-1α Induction in Human Keratinocytes (HaCaT): An In Vitro Model for Chemoprevention in Skin

Table 1

Effect of different incubation times and PMA concentrations on the intracellular production of IL-1α.
PMA conc. (ng/mL)Ctrl.6.2512.52550100
Exposure (h)IL-1α concentration (pg/mL)
6 1 6 8 . 8 5 ± 3 2 . 5 7 a A 1 3 2 . 7 5 ± 6 . 0 7 a A 2 0 3 . 9 7 ± 8 . 9 7 a A 1 8 0 . 1 6 ± 1 2 . 2 7 a A 1 8 9 . 3 8 ± 1 6 . 3 6 a A 1 6 2 . 6 6 ± 2 7 . 7 6 a A
12 1 1 2 . 5 9 ± 0 . 8 3 a B 6 0 . 6 4 ± 1 0 . 4 9 b B 7 1 . 2 2 ± 8 . 9 9 B a b 8 6 . 8 5 ± 1 1 . 7 1 B a b 7 5 . 0 6 ± 9 . 9 0 B a b 7 3 . 7 3 ± 4 . 0 7 B a b
24 1 5 . 6 9 ± 1 . 1 5 a C 9 . 6 1 ± 0 . 4 8 a C 1 0 . 2 9 ± 0 . 6 6 a C 9 . 8 2 ± 2 . 4 8 a C 9 . 1 5 ± 1 . 3 8 a C 9 . 6 1 ± 0 . 3 7 a C
Cells were seeded at a density of 15 000 cells/well. Values represent the mean ± standard deviation of four replicates. Letters in superscript (row) indicate significant different between control and PMA concentrations; letters in subscript (column) indicate significant difference between incubation periods. Values were considered significant when 𝑃 < 0 . 0 5 .