Journal of Skin Cancer

Research Article

Interleukin-1α Induction in Human Keratinocytes (HaCaT): An In Vitro Model for Chemoprevention in Skin

Table 2

Effect of PMA on the intracellular and extracellular IL-1α production and determination of cytotoxic effects.
PMA conc. (ng/mL)Ctrl.6.2512.52550100
IL-1α concentration (pg/mL)
Intracellular 2 5 6 . 3 0 ± 2 6 . 2 4 a A 2 6 6 . 7 1 ± 2 5 . 0 6 a A 2 2 3 . 2 9 ± 8 2 . 1 4 a A 2 0 0 . 9 1 ± 6 9 . 9 7 a A 2 3 4 . 7 3 ± 3 1 . 1 6 a A 2 3 3 . 1 7 ± 3 6 . 3 8 a A
Extracellular 1 . 7 4 ± 0 . 2 3 a B 2 . 6 0 ± 0 . 8 7 a B 1 . 7 8 ± 0 . 6 9 6 a B 2 . 2 8 ± 0 . 8 1 a B 1 . 8 6 ± 0 . 5 0 a B 1 . 9 3 ± 0 . 4 0 a B
% LDH release 𝟕 . 𝟏 𝟐 ± 𝟎 . 𝟗 𝟑 𝐚 𝟕 . 𝟗 𝟎 ± 𝟏 . 𝟔 𝟓 𝐚 𝟔 . 𝟗 𝟏 ± 𝟎 . 𝟔 𝟖 𝐚 𝟗 . 𝟒 𝟖 ± 𝟐 . 𝟔 𝟕 𝐚 𝟔 . 𝟓 𝟐 ± 𝟎 . 𝟔 𝟓 𝐚 𝟕 . 𝟐 𝟐 ± 𝟎 . 𝟔 𝟗 𝐚
Cells were seeded at a density of 3 0 × 1 0 4 /well and incubated for 6 h. Values represent the mean ± standard deviation of 6 replicates. Cytotoxicity represents LDH release as a percentage of the total. Letters in superscript indicated significant differences between control and PMA concentration; letters in subscript indicated significant differences between intracellular and extracellular IL-1α production. 𝑃 < 0 . 0 5 was considered significant. PMA: phorbol 12-myristate 13-acetate; LDH: lactate dehydrogenase.