Interleukin-1α Induction in Human Keratinocytes (HaCaT): An In Vitro Model for Chemoprevention in Skin
Table 3
UV-induced IL-1α production, cytotoxic, and apoptotic effects.
Incubation period
Unit of measure
UV dose (mJ/cm2)
Ctrl
20
40
80
160
240
6 h
icIL-1α (pg/mL)
icIL-1α fold increase
exIL-1α (pg/mL)
% LDH release
Caspase-3 (fold increase)
12 h
icIL-1α (pg/mL)
icIL-1α fold increase
exIL-1α (pg/mL)
% LDH release
Caspase-3 (fold increase)
24 h
icIL-1α (pg/mL)
icIL-1α fold increase
exIL-1α (pg/mL)
% LDH release
Caspase-3 (fold increase)
Cells were seeded at a density of /well; values represent mean ± standard deviation of quadruplicates; small letters in superscript indicates difference between control and UV dose (mj/cm2); capital letter, in subscript indicates significant difference between intracellular IL-1α and extracellular IL-1α (pg/mL); small letter in subscript indicate significant difference between incubation periods (hours) for icIL-1α (pg/mL), icIL-1 fold increase, toxicity, and capsase-3; Values were considered significant if . icIL-1α: intracellular interleukin 1α; exIL-1α: extracellular interleukin 1α; LDH: lactate dehydrogenase.