Interleukin-1α Induction in Human Keratinocytes (HaCaT): An In Vitro Model for Chemoprevention in Skin

Table 4

Effect of dexamethasone and ibuprofen on icIL-1α production, cell viability and cytotoxicity.

Dexamethasone conc (mM)	Ctrl	Positive Ctrl	1.25	0.63	0.31
icIL-1α (pg/mL)	19.40±2.50^a	76.04±7.20^b	48.90±9.81^ca	67.70±10.40^ba	67.60±9.22^ba
(% inhibition)	—	—	40.77±5.40	15.41±7.80	17.05±12.02
% ATP production	100.00±3.25^aa	85.65±6.12^ba	76.39±6.09^ba	78.69±4.63^ba	86.59±3.37^ba
% LHD release	3.48±0.83^aa	8.84±0.96^ca	5.51±0.41^ba	5.49±0.92^ba	4.45±0.71^ba
Ibuprofen conc (mM)	Ctrl*	Positive Ctrl*	1.25	0.63	0.31
icIL-1α (pg/mL)	—	—	20.67±3.69^ab	86.84±11.27^bb	86.45±12.77^bb
(% inhibition)	—	—	72.89±5.60	(—)	(—)
% ATP production	100.00±3.80^aa	90.19±9.38^aa	64.76±5.89^bb	84.75±11.99^ba	88.98±14.01^aa
% LDH release	4.65±0.19^aa	10.17±0.84^ca	5.77±0.59^ba	5.30±0.55^aab	4.75±0.72^aab

Ctrl-control represents cells that have not been exposed to UV-B light. Values presented are the mean ± standard deviations. letters in subscript indicate significant difference between treatment concentration of dexamethesone and ibuprofen; values in italics represent % inhibition of icIL-1α by dexamethasone and ibuprofen (—) indicates no inhibition; % inhibition calculated from the positive control. Effect of dexamethasone and ibuprofen was conducted in the same experiment therefore, similar control and pos control were used. *Pos Ctrl- Positive control represents cells exposed to 80 mj/cm² of UV-B light. icIL-1α intracellular interleukin-1alpha.