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Spectroscopy
Volume 24 (2010), Issue 3-4, Pages 325-331
http://dx.doi.org/10.3233/SPE-2010-0446

Emission lifetime study of fluorescence probes based on G-quadruplex oligonucleotides end-labeled with pyrene moieties

A. Cupane,1 A. Dembska,1 T. Pedzinski,1 S. Takenaka,2 and B. Juskowiak1,3

1Faculty of Chemistry, A. Mickiewicz University, Poznan, Poland
2Department of Applied Chemistry, Kyushu Institute of Technology, Kitakyushu, Japan
3A. Mickiewicz University, Department of Chemistry, ul. Grunwaldzka 6, 60-780 Poznan, Poland

Copyright © 2010 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Fluorescence lifetime study of two probes abbreviated as Py-Htelom-Py and Py-TBA-Py, carrying pyrene moieties at both termini and sequences of Human telomere and Thrombin Binding Aptamer, respectively are reported. The effect of potassium ion on the photophysical processes was examined in order to elucidate factors that facilitate the production of excimer emission. Emission kinetics data indicated that the relative orientation of pyrene and neighboring nucleobase (guanine, adenine, thymine) plays a crucial role in determining both the rate of electron-transfer quenching of pyrene excited state and the efficiency of excimer emission.