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Spectroscopy
Volume 24 (2010), Issue 1-2, Pages 165-168
http://dx.doi.org/10.3233/SPE-2010-0417

Interaction between linker histone H1 and non-histone chromatin protein HMGB1

Alexander V. Fonin,1,2,3 Olga V. Stepanenko,1 Irina M. Kuznetsova,1 Konstantin K. Turoverov,1 Elena I. Kostyleva,1 and Vladimir I. Vorobyev1

1Institute of Cytology of the Russian Academy of Sciences, St. Petersburg, Russia
2St. Petersburg State Politechnical University, St. Petersburg, Russia
3Institute of Cytology of the Russian Academy of Sciences, Tikhoretsky av., 194064 St. Petersburg, Russia

Copyright © 2010 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The possibility of interaction between linker histone H1 and non-histone chromatin protein HMGB1 was studied by intrinsic UV-fluorescence, far and near-UV CD and light scattering. The obtained data allow us to assume that the increase of histone H1 content in the HMGB1 solutions in a low ionic strength is accompanied by the destruction of HMGB1 associates. The interaction between proteins causes the increase of ordered regions in the protein molecules and the minor changes in their tertiary structure.