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Spectroscopy
Volume 24 (2010), Issue 1-2, Pages 55-60
http://dx.doi.org/10.3233/SPE-2010-0404

Monitoring of metabolism perturbation in prostate PC-3 cancer cells by sub-lethal concentrations of methotrexate

Allison Derenne,1 Régis Gasper,1 and Erik Goormaghtigh1,2

1Laboratory for the Structure and Function of Biological Membranes, Center for Structural Biology and Bioinformatics, Université Libre de Bruxelles, Brussels, Belgium
2Laboratory for the Structure and Function of Biological Membranes, Center for Structural Biology and Bioinformatics, Campus Plaine CP206/02, Université Libre de Bruxelles, Bld du Triomphe 2, CP206/2, B1050 Brussels, Belgium

Copyright © 2010 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Infrared imaging was used to investigate the effect of sub-lethal concentration of methotrexate on prostate PC-3 cancer cells. Cells were exposed for 0, 24 and 48 h to 9 nM of methotrexate, the concentration that inhibits 50% of cell growth after 72 h. Sub-lethal concentrations of methotrexate induced a reproducible signature that can be measured by infrared spectroscopy. Student t-test revealed that the process occurs in at least two steps, the first one affecting the nucleic acid region of the spectrum and the second one, the protein region. Imaging of individual cells demonstrated that the recorded differences are not due to a specific subpopulation of cells but homogenously affects all the cells. Near perfect identification of treated cells could be obtained using a supervised classification.