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Spectroscopy
Volume 25 (2011), Issue 5, Pages 207-216
http://dx.doi.org/10.3233/SPE-2011-0510

Infrared microspectroscopic imaging as a probing tool to fast distinguish chemical compositions in calcified deposits of prostatic calculi and calcific tendonitis

Shan-Yang Lin,1 Tieh-Kang Wu,1 Hong-Jen Chiou,2 Ted Hueih-Shing Hsu,3 and Chih-Cheng Lin1

1Department of Biotechnology, Yuanpei University, Hsin-Chu, Taiwan, China
2Department of Radiology, Taipei Veterans General Hospital, and School of Medicine, National Yang-Ming University, Taipei, Taiwan, China
3Division of Urology, Department of Surgery, Lotung Pohai Hospital, Lotung, Ilan, Taiwan, China

Copyright © 2011 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The specificity and homogeneity of the real compositional components within the calcified deposits of prostatic calculi and calcific tendonitis were investigated using Fourier transform infrared (FT-IR) microspectroscopy with or without automatic imaging system. The second-derivative analysis was also applied to differentiate the overlapping components of individual spectra for the calcified samples. The FT-IR microscopic imaging results of present study indicate that the complicated components such as protein, type B or type A carbonated apatite, brushite and calcium oxalate monohydrate were contained in the calcified tissue of prostatic tissue, but the protein, type A and type B carbonated apatites were mainly included in the calcific tendonitis. However, the traditional manually single-point FT-IR spectral result only reveals a little component contained in the calcified tissues, leading to an inaccurate diagnose of the complicated components in the calcified mixture.