Application of FTIR spectroscopy coupled with chemometrics for authentication of <i>Nigella sativa</i> seed oil

Nurrulhidayah, A. F.; Man, Y. B. Che; Al-Kahtani, H. A.; Rohman, A.

doi:https://doi.org/10.3233/SPE-2011-0509

Journal of Spectroscopy

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Volume 25 | Article ID 470986 | https://doi.org/10.3233/SPE-2011-0509

Application of FTIR spectroscopy coupled with chemometrics for authentication of Nigella sativa seed oil

A. F. Nurrulhidayah,¹Y. B. Che Man,^1,2,3,6H. A. Al-Kahtani,³and A. Rohman^4,5

Abstract

The present study is intended to analyze the presence of grape seed oil (GSO) in Nigella sativa L. seed oil (NSO) using Fourier transform infrared (FTIR) spectroscopy and gas chromatography (GC). FTIR spectroscopy coupled with multivariate calibration of partial least square can quantify the levels of GSO in NSO at wavelength number of 1114–1074, 1734–1382 and 3005–3030 cm^–1. The coefficient of correlation (R²) obtained for the relationship between actual (x-axis) and FTIR predicted (y-axis) values are 0.981. The errors in cross validation and in prediction are 2.34% (v/v) and 2.37% (v/v), respectively.

Copyright

Copyright © 2011 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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