Spectroscopic Study of the Interaction of DNA with the Linker Histone H1 from Starfish Sperm Reveals Mechanisms of the Formation of Supercondensed Sperm Chromatin

Figure 3

Derivative melting curves of the complexes of DNA with the linker histone H1 from starfish sperm in 0.25 mM EDTA at different protein to DNA ratios (𝑟): DNA (dash); (1) DNA-H1Z, 𝑟=0.5; (2) DNA-H1Z, 𝑟=1.0; (3) DNA-H1Z, 𝑟=1.5; (4) DNA-H1T, 𝑟=1.0.