Spectroscopic Study of the Interaction of DNA with the Linker Histone H1 from Starfish Sperm Reveals Mechanisms of the Formation of Supercondensed Sperm Chromatin

Table 1

The main thermodynamic characteristics of the temperature-induced conformational transition of free DNA and of the DNA in the complexes with H1 starfish sperm (DNA-H1Z) and somatic H1 (DNA-H1T) in 0.25 mM EDTA at the different ratios of protein amino acid residues to DNA phosphates.

	DNA	DNA-HIZ 𝑟=0.5	DNA-HIZ 𝑟=1.0	DNA-HIZ 𝑟=1.5	DNA-HIT 𝑟=1.0
Hyperchromiceffect 𝐺, %	35.1±0.5	39.8±0.5	39.2±0.5	35.7±0.5	39.3±0.5
𝑇𝑚𝐼, °C	45.0±0.5	45.0±0.5	45.0±0.5	45.0±0.5	45.0±0.5
𝑇𝑚𝐼𝐼, °C	—	77.0±0.5	77.0±0.5	77.0±0.5	73.0±0.5
Fraction of DNA Melted at 𝑇𝑚𝐼, %	100±0.5	71.0±0.5	54.0±0.5	37.0±0.5	51.0±0.5
Fraction of DNA Melted at 𝑇𝑚𝐼𝐼, %	0	29.0±0.5	46.0±0.5	63.0±0.5	49.0±0.5