Phosphorus Compartmentalization on the Cellular Level of Douglas Fir Root as Affected by Mn Toxicity: A Synchrotron-Based FTIR Approach
Figure 2
((A), (a)) Synchrotron-based FTIR microscopy of 10 μm-thick freeze-dried roots cross-section of Douglas fir var. glauca of control and 5 mM Mn-treated plants, respectively. The scanned regions of interest for the spectromicroscopy maps were chosen from online visible microscopic pictures (marked with the red rectangle in (B), (b)). An area was mapped with full high-resolution spectra in each pixel. 2D ((A), (a)) and 3D ((D), (d)) images were generated for the absorption line of phosphorus group (P=O, phosphate and phosphine oxide) at 1100 cm−1. The cumulative FTIR absorption spectra of freeze-dried roots sections as recorded in reflection mode by the SR-FTIR spectromicroscope of ID21 at ESRF are shown in (C) and (c).
