A Comparison between Vanadyl, Vanadate, and Decavanadate Effects in Actin Structure and Function: Combination of Several Spectroscopic Studies
Figure 2
G-actin intrinsic fluorescence. G-actin (5 μM) was incubated with VOSO4 (▲) or decavanadate () for 20 min, at 25°C, in 2 mM Tris-HCl (pH 7.5), 0.2 mM CaCl2. The maximum of intrinsic fluorescence spectra ( = 295 nm) were plotted against vanadyl or decavanadate (total) concentrations, considering 1.00 the value for native actin. The results shown are the average of, at least, triplicated measurements.
