Raman Microspectroscopy of the Yeast Vacuoles

Figure 1

Intracellular structures apparent on the video image of Candida yeast and respective spatially-resolved Raman spectra of cultivation medium (A), vacuole (B), protein structure I (C), protein structures II (D), and lipidic structure (E). Spectra are normalized using intensity of the water OH-stretching band at 3400 cm−1 as an intensity standard. Raman spectrum of 2 M aqueous solution of polyphosphate is shown to highlight its contribution to the spectrum of vacuole.
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