Research Article

Identification of Contaminated Cells with Viruses, Bacteria, or Fungi by Fourier Transform Infrared Microspectroscopy

Table 1

Changes in peaks position at 857 and 1650 cm−1 as a result of Vero cells infection with HSV-1 or contamination with E. coli or fungi.

SampleWavenumber (cm−1)

Vero1650 ± 1.2857 ± 0.6
Fungi 1651 ± 1.1858 ± 1.1
Bacteria1657 ± 1.6857 ± 0.9
Vero + bacteria1656 ± 1.8856 ± 0.5
Vero + fungi1651 ± 2.1857 ± 0.8
Vero + HSV1649 ± 1.2853 ± 0.5

Vero cells were infected with 1 moi of HSV-1 or contaminated with E. coli or fungi. At 24 h later the different cell cultures were examined by FTIR microscopy. The results are means ± SD, ( ).