Singular Value Decomposition and Ligand Binding Analysis

Figure 9

Scatchard plot of Daun-DNA binding. The squares and circles show SVD-based fitting and a traditional Scatchard plot, respectively. The standard noncooperative McGee and von Hippel model fitting parameters are 𝐾=8.5±0.2×10⁵ M⁻¹ and 𝑛=3.14±0.06 bp/(bound drug molecule) while the SVD-based parameters for fitting are 𝐾=7.79±0.05×10⁵ M⁻¹ and 𝑛=3.0 bp/(bound drug molecule).